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trypsin edta 0.5

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O-064121 GIBCO Trypsin Chart.indd

Stable Trypsin. Replacement. Enzyme (1X). Trypsin, 0.25% (1X). Trypsin, 2.5% (10X). Trypsin-EDTA. (0.05% Trypsin,. EDTA•4Na) (1X). Trypsin-EDTA (0.5% … http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf.Par.60667.File.dat/0_064121_GIBCO_Trpsn_Crt2.pdf
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Trypsin-EDTA solution (T3924) – Product Information Sheet

by P Parenzyme – Related articles http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/t3924pis.pdf
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Life Science Reagents for RNAi Research

Trypsin-EDTA solution 1 ×, 0.5 g porcine trypsin and 0.2 g EDTA. • 4Na per liter of Hanks Balanced Salt Solution with phenol red., … http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/biobasics_rnai.pdf
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Calverial cell Preparation Reagents: PBS: PBS (Ca, Mg free) + P/S …

Trypsin-EDTA: (0.5%/0.2%): GIBCOBRL cat# 25300-054. Frozen media: 80% FBS + 20% DMSO keep at 40C all the time. Put frozen box at refrigerator before start. … http://csdb.nidr.nih.gov/methods/Calvaria prep.pdf
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II.C.3 DEVELOPMENT OF STABLE TRANSFECTED CELL LINE Materials …

Transfer 0.5 mL cell suspension into 24-well plate containing 500 µl of (media + … Add 100µl of trypsin-EDTA, pipeting several times gently to … http://www.biochem.northwestern.edu/morimoto/research/Protocols/II. Eukaryotes/C. Stable Cell Lines/2. Gen’s protocol.pdf
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Studying Early Hematopoiesis Using Avian Blastoderm Cultures

a final concentration of 0.5 g trypsin and 0.2 g EDTA-4Na per liter. … 10 min and resuspend in 1X trypsin-EDTA (0.5 mL per 10 fragments). After a 10 min … http://www.springerlink.com/index/t223k56v2342p180.pdf
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Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of …

freeze at –20°C. Prepare 20X (20 mM) stock solution EDTA from a 0.5-M stock solu- tion (a 1:25 dilution—see Note 1), dilute trypsin and EDTA to 1X in PBS, … http://www.springerlink.com/index/v37072401q46t32r.pdf
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The Amine-Containing Cutaneous Irritant Heptylamine Inhibits the …

incubated in HBSS supplemented with trypsin-EDTA (0.5 mg/ml;. Invitrogen) for 20 min at 37°C. The resulting epidermal cell suspen- … http://molpharm.aspetjournals.org/content/71/6/1685.full.pdf
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Promotion by Verapamil of Vincristine Responsiveness in Tumor Cell

0.5 ml of 0.05% trypsin:EDTA. PBS (2 ml) containing 2% fetal bovine … times with cold PBS (5 ml), trypsinized (0.05% trypsin:EDTA, 0.5 ml), … http://cancerres.aacrjournals.org/cgi/reprint/43/2/808.pdf
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Interaction of Vasopressin and Oxytocin with Human Breast …

until confluent and removed with trypsin-EDTA (0.5 g/liter trypsin-. 0.2 g/liter EDTA) and replated on 35-mm Petri dishes in DMEM with … http://cancerres.aacrjournals.org/cgi/reprint/50/24/7882.pdf
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Trypsin-EDTA

Trypsin 1:250. Trypsin/EDTA Trypsin/EDTA. (10X). 1% in PBS. 0.25% in PBS. 2.5% in PBS. 0.05/0.02% in. PBS. 0.5/0.2% in. PBS w/o Ca2+, Mg2+ w/o Ca2+, Mg2+ … http://www.autogenbioclear.com/L2143.pdf
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Intercellular Calcium Waves in Cultured Enteric Glia From Neonatal …

by W Zhang – 2003 – Cited by 23 http://deepblue.lib.umich.edu/bitstream/2027.42/35024/1/10215_ftp.pdf
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Isolation and Characterization of Dermal Lymphatic and Blood …

incubated for 7 min at 37 C in trypsin/EDTA (Life Technolo- gies). Cell pellets were resuspended in ice-cold PBS/2 mM. EDTA/0.5% BSA (Sigma-Aldrich), … http://www.jem.org/cgi/reprint/194/6/797.pdf
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Culturing JM8 and JM8

0.5 ml. Sterile filter through 0.2µM filter unit. 0.1% Trypsin. â— To 475ml PBS add 0.1g EDTA and 0.5g D-glucose. â— Add 5ml Chicken Serum. … http://www.komp.org/pdf/Culturing_and_media_conditions_for_KOMP_CSD_JM8_and_JM8_F6_ES_cell_clones_Protocol.pdf
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Culturing JM8 and JM8

To 475ml PBS add 0.1g EDTA and 0.5g D-glucose. â— Add 5ml Chicken Serum. â— Add 20ml 2.5% Trypsin. â— Filter sterilize (0.22 µm). … http://www.komp.org/pdf/Culturing_and_media_conditions_for_KOMP_CSD_JM8_N4_ES_cell_clones_Protocol.pdf
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THE EFFECT OF INDOMETHACIN AND ASPIRIN ON ALKALINE PHOSPHATASE …

was washed twice with 5 ml trypsin EDTA (0.5 and 0.2 g/1 respectively) at 37°C until the cells became detached (approximately 5 min). Cell … http://rheumatology.oxfordjournals.org/cgi/reprint/27/4/291.pdf
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R etinal pigment epithelial [RPE] cells play a major

by JY Guo’Xing Xu – 2004 – Related articles http://qikan.shouxi.net/upload/pdf/153/1569/93162_6944.pdf
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Trabecular meshwork (TM) tissue is located at the angle of

and trypsin-EDTA (0.5% trypsin; 5.3 mM EDTA, tetrasodium salt) were obtained from Gibco BRL (Rockville, MD). Acid-solubilized porcine … http://www.iovs.org/cgi/reprint/43/11/3465.pdf
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Intraocular pressure (IOP) is determined by the balance be-

trypsin-EDTA (0.5% and 5.3 mM [tetrasodium salt], respectively] were obtained from Invitrogen-Gibco (Rockville, MD); acid-solubilized type … http://www.iovs.org/cgi/reprint/44/10/4331.pdf
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endothelium via iC3b Neutrophil adhesion to xenogeneic

FCS, trypsin-EDTA (0.5% trypsin + 0.53 mM. L-glutamine. and penicillin/streptomycin were obtained from Hazel- ton Biologics, Inc. (Lenexa, KS]. … http://www.jimmunol.org/cgi/reprint/146/2/730.pdf
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Phagocytosis of Crocidolite Asbestos Induces Oxidative Stress, DNA …

trypsin (0.25%, wt/vol) and EDTA (0.5 mM) for 10 min with gen- tle rotation on a shake table at RT to remove the adherent fibers. Once washed with PBS, … http://www.ajrcmb.org/cgi/reprint/23/3/371.pdf
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Renibacterium salmoninarum grown in cell

monolayer by treatment with trypsin-EDTA (0.5 ml per flask) followed by the addition of 2 ml volumes of fresh cell culture medium to each flask. … http://mic.sgmjournals.org/cgi/reprint/143/10/3349.pdf
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G ENAXXON

0.5 g/l Trypsin. 0.2 g/l EDTA. pH 7.4 – 7.6. Activity. 1 g of Trypsin digests. 250 g of Casein substrate. 1 g of Trypsin digests. 250 g of Casein substrate … http://www.genaxxon.com/docs/pdf/trypedta.pdf
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CMMT lnvitrogen Supply Center Pricelist

(0.5% Trypsin with EDTA 4Na) 10X. 100 .ml. 25200-056 Trypsin-EDTA (0.25% Trypsin with EDTA 4Na) 1X. 100 ml. 25200-072 Trypsin-EDTA (0.25% Trypsin with EDTA … http://www.cmmt.ubc.ca/sites/default/files/pdf_products_invitrogen.pdf
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Thank you for your confidence in our products and services.

15160-047. 39.96. Antibiotic/Antimycotic Solution (100X), liquid. 100 ml. 15240-062. 64.60. 37.94. Trypsin-EDTA (10X), liquid, 0.5% Trypsin, 5.3mM. EDTA•4Na … http://www.aul.uzh.ch/materialzentrum/liefervereinbarungen/invitrogenkontrakt-1.pdf
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Maintenance of HFE-producing CHO cells

3) Add about 0.5-1.0 ml of Trypsin/EDTA solution to the cells, making sure that all the cells on the plate are covered with this solution, and incubate for … http://www.its.caltech.edu/~bjorker/Protocols/Maintenance_of_HFE_prod _ce.pdf
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Normal Human Mammary Epithelial Cells

Insulin, 0.5 ml. Trypsin/EDTA , 100 ml. CC-5034. ReagentPack. HEPES buffered Saline Solution,100 ml. Trypsing Neutralizing Solution, 100 ml … http://genome.ucsc.edu/ENCODE/protocols/cell/HMEC_Bernstein_protocol.pdf
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The Modulation of Tissue Factor by Endothelial Cells during Heat …

21 Nov 2009 … ciently, the trypsin/EDTA protocol was used for the flow cytometry experiments. HUVEC monolayers were washed with PBS containing. 0.5 mM … http://www.jbc.org/content/278/13/11065.full.pdf
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Intracellular Actions of Group IIA Secreted Phospholipase A2 and …

30 Nov 2009 … Trypsin/EDTA (0.5 ml) was added to the cell pellet in the well, and the plate was placed in the incubator for about 30 min. … http://www.jbc.org/content/281/24/16245.full.pdf
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Downstream effects on human low density lipoprotein of …

with trypsin/EDTA (0.5 g/l trypsin and 0.02% EDTA in Hank’s buffered saline solution) and split to produce 8 105 cells per … http://www.jlr.org/cgi/reprint/46/3/484.pdf
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Downstream effects on human low-density lipoprotein of …

confluence (5-7 days), they were passaged by treatment with trypsin/EDTA (0.5 g/l trypsin and 0.02% EDTA in Hank’s Buffered Saline Solution), … http://www.jlr.org/cgi/reprint/M400339-JLR200v1.pdf
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In Vivo and In Vitro Measurement of Apoptosis in Breast Cancer …

by DJ Yang – 2001 – Cited by 71 http://www.cellpointweb.com/pdf/annexin Nov 1 2001.pdf
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Src-dependent ezrin phosphorylation in adhesion-mediated signaling.

Cells were detached by limited trypsin/EDTA, treated with soybean trypsin inhibitor. (0.5 mg/ml), and resuspended in serum free DMEM containing heat … http://www.molbiolcell.org/cgi/reprint/E04-08-0721v1.pdf
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SUBSTRATE-ATTACHED GLYCOPROTEINS MEDIATING ADHESION OF NORMAL AND …

bottles by using a trypsin (0.05% wt/vol Difco trypsin. I:250)-EDTA (0.5 mM) solution; and were screened for and found to be free of Mycoplasma with a … http://jcb.rupress.org/cgi/reprint/63/1/71.pdf
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Relationship between functional Na+ pumps and mitogenesis in …

trypsin-EDTA, pelleted, and resuspended at a density of 0.5-1.0 x lo6 cells/50 ~1 of 40 mM trisodium citrate (pH 7.6), 250 mM … http://ajpcell.physiology.org/cgi/reprint/264/1/C169.pdf
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Controlling the in vitro differentiation of embryonic stem cells …

by DB Ou – Related articles http://www.cellbiolint.org/cbi/imps/pdf/CBI20090021.pdf
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Tissue-engineered intrasynovial tendons: Optimization of …

by AY Zhang – Related articles http://www.rehab.research.va.gov/jour/09/46/4/pdf/zhang.pdf
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PII: S0248-4900(94)80022-7

by A Venant – 1994 – Cited by 9 http://www.biolcell.org/boc/082/0195/boc0820195.pdf
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Impact of Heterogeneity within Cultured Cells on Bacterial …

in tissue-culture ffasks by treatment with trypsin-EDTA (0.5% trypsin and 0.53. mM EDTA in Hanks’ balanced salt solution), washed, counted, seeded into … http://iai.asm.org/cgi/reprint/68/2/861.pdf
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347-350 Enzymes

0.5 g/L Porcine Trypsin (tested for porcine parvo virus) 1 mM EDTA•4Na in. Hanks’ Balanced Salt Solution (Ca++ and Mg++ free). … http://www.biowish.com/system/uploadtechN/FD29O5KDM.pdf
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HUMAN CYTOGENETICS

Trypsin EDTA (0.5%), EDTA 0.2% , 10X Conc. Product Name. Catalogue Unit. No. Size. Colchicine Solution, 10µg/ml in DPBS. 12-003-1C 25ml … http://www.lifetechindia.com/Human_Cytogenetics_Prenatal_Karyotyping_Bone_Marrow_Peripheral_Blood_Life_Technologies_India.pdf
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Loperamide: A positive modulator for store-operated calcium channels?

by JL HARPER – 1997 – Cited by 16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC25137/pdf/pq014912.pdf
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Generation of purified oligodendrocyte progenitors from embryonic …

trypsin-EDTA (Sigma, 0.5 mg/ml), followed by treatment with soybean trypsin inhibitor (Sigma,. 0.5 mg/ml). They were concentrated in Hanks’ buffered salt … http://www.fasebj.org/cgi/reprint/04-1931fjev1.pdf
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RESGROâ„¢ Culture Medium 250 mL

Add 0.5 mL of trypsin/EDTA to the cell pellet. 7. Pipette up and down with a 1 mL pipette (do not use pipette tip of smaller volume). … http://www.millipore.com/publications.nsf/a73664f9f981af8c852569b9005b4eee/979fb6bfed6a20b48525730600751663/$FILE/SCM001.pdf
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Technical paper of TX-WES cell culture medium

add 0.5 ml trypsin-EDTA (Invitrogen Cat. Nr.25200-056) on the 6-well. • remove the trypsin immediately. • add again 0.5 ml trypsin-EDTA on the 6-well … http://search.cosmobio.co.jp/cosmo_search_p/search_gate2/docs/THX_/TXWES25.20071109.pdf
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Multiparameter In Situ Analysis of Trophoblast Cells in Mixed Cell …

1 x trypsin-EDTA (0.5% trypsin, 0.2% EDTA; Gibco) at 3 7 T , followed by a 2-hr incubation in collagenase (1 mg/ml Type 11; Sigma, Bornem, Bel- … http://www.jhc.org/cgi/reprint/43/7/709.pdf
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Chick Tissue Culture: reference- Growth plate chondrocyte …

fetal calf serum, 0.02% EDTA pH 7.6 for isolate muscle and cartilage-0.25%trypsin, or containing collagenase – 0.5 ml per sample of muscle or cartilage … http://www.csun.edu/~vcbio001/tiscult.pdf
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Ej0838 493..499

detached using trypsin–EDTA (0.5g/l trypsin, 0.2g/l. EDTA) and used at passage 1. Each co-culture experi- ment was performed with a fresh isolate of primary … http://www.eje-online.org/cgi/reprint/142/5/493.pdf
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The effect of mouse embryonic fibroblast in direct differentiation …

by M Hashemi-Tabar – 2007 – Related articles http://www.ijrm.ir/library/upload/article/29-33.pdf
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Basic Protocol for Primary Mammalian Endothelial Cells

trypsin. If necessary, prolong the incubation time for two more minutes at 37°C or add trypsin 0.5% – EDTA 0.2%. Once the majority of cells (>90%) have been … http://www.lonzabio.com/fileadmin/groups/marketing/Downloads/Protocols/Primary_cells/amaxa_OP_Basic_Endothelial_VPI-1001.pdf

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